Dr. Ross Carlson, Chemical and Biological Engineering July 2009 - February 2014
Montana State University
My work here is part of a broader study of Escherichia coli metabolism intended to improve predictive interpretation of biological networks. In this position I worked with a doctoral student providing my expertise in microbiology and proteomics. This project includes characterization of overflow metabolism, protein expression and biomass composition of cells grown in chemostats under iron, carbon and nitrogen limitation. In addition, I worked with a graduate student on efforts to improve the labs computational capability for the calculation of the elementary flux mode set for biological networks. Several papers from this work are in preparation. I also managed lab inventory by ordering supplies.
Dr. Philip Stewart, Center for Biofilm Engineering June 2007 - June 2009
Montana State University
This was two one year projects. The first examined the transcriptomic response of Pseudomonas aeruginosa biofilms exposed to antibiotics. This project included PCR, and RNA extraction. The second project screened a novel set of antimicrobials collectively known as bismuth-thiols, for the purpose of identifying those most effective against biofilms.
Dr. Joseph Frank, Department of Food Science and Technology January 2007 - May 2007
University of Georgia
This was a six month project to study survival of Escherichia coli O157:H7 on leaf lettuce under abuse and regular storage conditions similar to harvest, transport, and shelf display storage. The project considered the conditions of wilting, internalization or surface localization as factors. I was trained on the multi-photon confocal at the Center for Ultrastructural Studies at UGA during this work.
Ph. D. in Food Science & Technology, University of Georgia, Athens, GA
M.S. in Food Science & Technology, University of Georgia, Athens, GA
B.S.A. in Food Science & Technology University of Georgia, Athens, GA
Folsom, J. P. and R. P. Carlson. 2014. Physiological, elemental composition, and proteomic analyses
of Escherichia coli ammonium-limited chemostat growth with comparison to iron- and glucose-limited chemostat growth.
Stewart, P. S., M. J. Franklin, K. S. Williamson, J. P. Folsom, L. Boegli and G. A. James. 2015. Contribution of stress responses to antibiotic tolerance in Pseudomonas aeruginosa biofilms. Antimicrobial Agents and Chemotherapy 59:3838-3847.
Folsom, J. P., A. E. Parker, and R. P. Carlson. 2014. Physiological and proteomic analysis of Escherichia coli iron-limited chemostat growth. Journal of Bacteriology 196:2478-2761.
Hunt, K. H., J. P. Folsom, R. L. Taffs, and R. P. Carlson. 2014. Complete enumeration of elementary flux modes through scalable demand-based subnetwork definition. Bioinformatics 30:1569-1578.
Folsom, J.P., B. Baker, and P. S. Stewart. 2011. In vitro efficacy of bismuth thiols against biofilms formed by bacteria isolated from human chronic wounds. Journal of Applied Microbiology 111:989-996.
Folsom, J.P., L. Richards, B. Pitts, F. Roe, G. D. Ehrlich, A. Parker and P. S. Stewart. 2010. Physiology of Pseudomonas aeruginosa in biofilms as revealed by transcriptional profiling. BMC Microbiology 10:294.
Folsom, J. P. and J. F. Frank. 2007. Proteomic analysis of a hypochlorous acid tolerant Listeria monocytogenes cultural variant exhibiting enhanced biofilm production. Journal of Food Protection 70:1129-1136.
Folsom, J. P. and J. F. Frank. 2006. Chlorine resistance of Listeria monocytogenes biofilms and relationship to subtype, cell density, and planktonic cell chlorine resistance. Journal of Food Protection 69:1292-1296.
Folsom, J. P., J. F. Frank, and G. R. Siragusa. 2006. Formation of biofilm at different nutrient levels by various genotypes of Listeria monocytogenes. Journal of Food Protection 69:826-834.
Folsom, J.P. and J.F. Frank. 2000. Heat inactivation of Escherichia coli O157:H7 in apple juice exposed to chlorine. Journal of Food Protection 63: 1021-1025.